FUNCTION OF COMPONENTS OF COMPLEMENT IN IMMUNE HEMOLYSIS
نویسندگان
چکیده
منابع مشابه
Function of Components of Complement in Immune Hemolysis
1. At a temperature of 1 degrees C., C'2, C'4, and C'1 combine with sensitized sheep erythrocytes, while C'3 does not combine. 2. C'1, although combining with sensitized cells in the absence of C'4, is hemolytically inert unless C'4 combines previous to, or simultaneously with it. 3. C'4 does not combine in the absence of C'1. 4. Although C'3 is not fixed by antibody-sheep cell aggregates, it i...
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15 صفحه اولMechanism of Hemolysis by Complement
1. Sensitization confers upon the red cell the property of adsorbing complement from solution. The submicroscopic film of immune serum protein deposited upon the cell surface during sensitization, and completely analogous to the precipitate formed in a soluble antigen-antibody reaction (e.g., sheep serum vs. rabbit anti-sheep serum) acts as absorbent, the degree of sensitization (size of the fi...
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An Improved Method for the Preparation of EAC14, an Intermediate in Immune Hemolysis by Carp Complement
We have succeeded in preparing EAC14 (sheep erythrocytes sensitized with carp antibody and complement components, Cl and C4) by incubating EA with carp serum in the presence of 5 mM TTHA (triethylenetetramine-N,N,N’,N’:N”:N”’hexaacetic acid), a selective chelator of Mg’+, and 5 mM CaCl* at pH 7.4. The optimal conditions for preparing EAC14 were as follows: ionic strength, 0.085; reaction temper...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1942
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.76.1.93